Temperature-sensitive Mutants of Fowl Plague Virus (Influenza A) Generated by Undiluted Passages at 33 C
نویسندگان
چکیده
منابع مشابه
Temperature-Sensitive Mutants of Measles Virus
were isolated after mutagenesis by 5-azacytidine, 5-fluorouracil, or proflavine. The restricted replication of all mutants at 39 C was blocked subsequent to cell penetration and could not be attributed to heat inactivation of virus infectivity. Complementation analysis was made possible through the use of poly-L-ornithine. The members of one complementation group exhibited wild-type RNA synthes...
متن کاملTemperature-Sensitive Mutants of Vesicular Stomatitis Virus
Different temperature-sensitive mutants of vesicular stomatitis virus have been characterized in terms of their ability to induce synthesis of viral ribonucleic acid (RNA) in BHK-21 cells at 39 C (the restrictive temperature for these mutants). Mutants belonging to complementation groups I and IV (and probably II) did not induce actinomycin-resistant RNA synthesis in infected cells incubated at...
متن کاملStructure and assembly of hemagglutinin mutants of fowl plague virus with impaired surface transport.
Five temperature-sensitive mutants of influenza virus A/FPV/Rostock/34 (H7N1), ts206, ts293, ts478, ts482, and ts651, displaying correct hemagglutinin (HA) insertion into the apical plasma membrane of MDCK cells at the permissive temperature but defective transport to the cell surface at the restrictive temperature, have been investigated. Nucleotide sequence analysis of the HA gene of the muta...
متن کاملIsolation and preliminary characterization of temperature-sensitive mutants of influenza virus.
Isolation of temperature-sensitive (ts) mutants was attempted from the WSN strain of influenza A virus which was grown and assayed in MDBK cells. After growth of wild-type virus in the presence of 5-fluorouracil, 15 ts mutants were selected for which the ratio of plaquing efficiency at 39.5 C to that at 33 C was 10(-3) or less. In pairwise crosses of ts mutants, recombination and complementatio...
متن کامل[Isolation of temperature-sensitive mutants of measles virus].
Measles virus differs from other members of paramyxoviruses in its prolonged growth cycle1), its degree of involvement of the cell nucleus2), its lack of neuraminidase3) and the sedimentability of its RNA4). We have undertaken a study of mutants of this virus in the hope that the study might circumvent some of the problems encountered with temperaturesensitive (ts) mutants of other paramyxoviru...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1983
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-64-12-2621